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qiagen 59104 甲基化转化试剂盒EpiTect Bisulfite Kit

  • 更新时间:  2023-07-26
  • 产品型号:  
  • 简单描述
  • qiagen 59104 甲基化转化试剂盒EpiTect Bisulfite Kit
    亚硫酸氢盐的*转化和DNA回收,用于甲基化分析
    简化的流程,获得快速、可靠的结果
    胞嘧啶*转化
    *的DNA保护体系,用于高度敏感的分析
    优化的试验方案可处理FFPE组织样本DNA
    离心柱和高通量96孔板方式
详细介绍

qiagen 59104 甲基化转化试剂盒EpiTect Bisulfite Kit

亚硫酸氢盐的*转化和DNA回收,用于甲基化分析

  • 简化的流程,获得快速、可靠的结果
  • 胞嘧啶*转化
  • *的DNA保护体系,用于高度敏感的分析
  • 优化的试验方案可处理FFPE组织样本DNA
  • 离心柱和高通量96孔板方式
  • EpiTect Bisulfite Kit可在6小时内将DNA 序列中非甲基化的胞嘧啶*转化为尿嘧啶。EpiTect 96 Bisulfite操作时间少于7小时。高灵敏度的方法采用全新的保护机制防止DNA降解,确保即使是1 ng的DNA也可获得灵敏的结果,转化率可高达99%。得益于*的DNA保护技术,EpiTect Bisulfite转化后DNA高度适用于EpiTect Whole Bisulfitome Kits转化后全基因组扩增。因此可在转化后DNA量较少的情况下确保可靠的扩增。EpiTect Bisulfite Kits包括离心柱或96孔板形式的扩增和脱磺酸基过程,确保快速可靠的实验结果用于下游应用。两个试剂盒都可用于离心机或真空装置,另外,EpiTect Bisulfite Kit可在QIAcube全自动核酸纯化仪上全自动运行。

qiagen 59104 甲基化转化试剂盒EpiTect Bisulfite Kit

Performance

快速得到实验结果

完整的EpiTect离心和真空过程只需6小时,包括从DNA到待分析的亚硫酸氢盐转化后的高纯DNA。EpiTect 96 Bisulfite操作时间少于7小时。传统的亚硫酸氢盐转化过程需要18个小时,且需要大量的操作(参见"快速简单的亚硫酸氢盐转化"和"Time saving procedure")。EpiTect Bisulfite Kit为成功进行亚硫酸氢盐转化和DNA回收提供所需的全部试剂。

快速简单的亚硫酸氢盐转化
 EpiTect流程传统流程
反应构建10分钟;预制溶液40分钟;需反应构建
亚硫酸氢盐转化5小时16小时
纯化30–45分钟; EpiTect离心柱或孔板120分钟;纯化、脱璜、沉淀和洗涤

订购信息:

EpiTect Bisulfite Kit (48)280059104
EpiTect 96 Bisulfite Kit (2)999059110
EpiTect Plus DNA Bisulfite Kit (48)225059124
EpiTect Whole Bisulfitome Kit (25)456059203
EpiTect MSP Kit (100)360059305
EpiTect MethyLight PCR Kit (200)1034059436
EpiTect HRM PCR Kit (100)144059445
EpiTect MethyLight PCR +ROX Kit (200)1060059496
EpiTect Control DNA (1000)273059568
EpiTect Control DNA, methylated (100)273059655
EpiTect Control DNA, unmethylated (100)273059665
EpiTect PCR Control DNA Set (100)546059695
EpiTect Fast 96 DNA Bisulfite Kit1091059720
EpiTect Fast 96 FFPE Bisulfite Kit1931059740
EpiTect Fast Bisulfite Kit (10)84759802
EpiTect Fast DNA Bisulfite Kit (50)243059824
EpiTect Fast DNA Bisulfite Kit (200)829059826
EpiTect Fast FFPE Bisulfite Kit (50)424059844
EpiTect Fast LyseAll Bisulfite Kit (50)272059864
EpiTect Fast LyseAll Bisulfite Kit (200)927059866
QIAamp DSP Virus Kit (50),CE452060704
QIAamp DSP DNA Blood Mini Kit (50), CE253061104
QIAamp DSP Virus Spin Kit (50)358061704
MagAttract HMW DNA Kit (48)397067563
DNeasy Plant Maxi Kit (24)459068163
DNeasy Plant Mini Kit (50)275069104
DNeasy Plant Mini Kit (250)1231069106
DNeasy 96 Plant Kit (6)1159069181
DNeasy Blood & Tissue Kit (50)214069504
DNeasy Blood & Tissue Kit (250)881069506
DNeasy mericon Food Kit (50)199069514
DNeasy mericon 96 QIAcube HT Kit (5)1143069571
DNeasy 96 Blood & Tissue Kit (4)878069581
DNeasy 96 Blood & Tissue Kit (12)2424069582
TissueLyser Single-Bead Dispenser, 5mm352069965
TissueLyser Single-Bead Dispenser, 7mm352069967
TissueLyser 3 mm Bead Dispenser, 96-well1016069973
TissueLyser 5 mm Bead Dispenser, 96-well1016069975
TissueLyser LT Adapter, 12-Tube1725069980
TissueLyser Adapter Set (2 x 24)1627069982
TissueLyser Adapter Set (2 x 96)1220069984
RNeasy Protect Animal Blood Kit (50)658073224
Rneasy Microarray Tissue Mini Kit (50)535073304
RNeasy Plus Universal Tissue Mini Kit50533073404
RNeasy Plus Universal Tissue Midi Kit10282073442
RNeasy FFPE Kit (50)581073504
RNeasy Micro Kit (50)660074004
RNeasy Plus Micro Kit (50)572074034
RNeasy Mini Kit (50)416074104
RNeasy Mini Kit (250)1752074106
RNeasy Mini QIAcube Kit (240)1928074116
RNeasy Protect Mini Kit (50)500074124
RNeasy Protect Mini Kit (250)2195074126
RNeasy Plus Mini Kit (50)476074134
RNeasy Plus Mini Kit (250)2006074136
RNeasy 96 QIAcube HT Kit (5)1487074171
RNeasy 96 Kit (4)2109074181
RNeasy 96 Kit (12)5797074182
Rneasy Plus 96 Kit (12)6681074192
RNeasy MinElute Cleanup Kit (50)472074204
RNeasy Protect Saliva Mini Kit (50)992074324
RNeasy FFPE Kit (50)594074404
RNeasy Protect Bacteria Mini Kit (50)657074524
RNeasy Protect Cell Mini Kit (50)500074624
RNeasy Fibrous Tissue Mini Kit (50)542074704
RNeasy Lipid Tissue Mini Kit (50)543074804
RNeasy 96 Universal Tissue Kit (4)2628074881
RNeasy Plant Mini Kit (20)209074903
RNeasy Plant Mini Kit (50)486074904
RNeasy Midi Kit (10)207075142
RNeasy Midi Kit (50)923075144
RNeasy Maxi Kit (12)490075162
exoEasy Maxi Kit (20)672076064
RNAlater (50ml)114076104

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