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CST spho-p38 MAPK (Thr180/Tyr182) (28B10) Mouse mA

  • 更新时间:  2017-12-11
  • 产品型号:  9216
  • 简单描述
  • CST spho-p38 MAPK (Thr180/Tyr182) (28B10) Mouse mAb
    Isotype:Mouse IgG1
    Applications:W IP IF-IC F
    Reactivity:H M R Mk Sc (Z)
    Sensitivity:Endogenous
    MW (kDa):43
详细介绍

CST spho-p38 MAPK (Thr180/Tyr182) (28B10) Mouse mAb

Applications Key: W=Western Blotting IP=Immunoprecipitation IF-IC=Immunofluorescence (Immunocytochemistry) F=Flow Cytometry
Reactivity Key: H=Human M=Mouse R=Rat Mk=Monkey Z=Zebrafish Sc=S. cerevisiae
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

9216:

Flow, Immunofluorescence, Immunoprecipitation, Western Blotting

Specificity / Sensitivity

Phospho-p38 MAPK (Thr180/Tyr182) (28B10) Mouse mAb detects p38 MAPK only when activated by dual phosphorylation at Thr180 and Tyr182. This antibody does not significantly cross-react with the corresponding phosphorylated forms of either p44/42 MAPK (Erk1/2) or SAPK/JNK. It does not detect nonphosphorylated p38 MAPK.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues around Thr180/Tyr182 of human p38 MAPK.

Western Blotting

Western Blotting

Western blot analysis of extracts from HeLa and NIH/3T3 cells, untreated or treated as indicated, using Phospho-p38 MAPK (Thr180/Tyr182) (28B10) Mouse mAb (upper) or p38 MAPK Antibody #9212 (lower).

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of Jurkat cells, untreated (green) or anisomycin-treated (blue), using Phospho-p38 MAPK (Thr180/Tyr182) (28B10) Mouse mAb compared to a nonspecific negative control antibody (red).

IF-IC

IF-IC

Confocal immunofluorescent analysis of HeLa cells either untreated (left) or anisomycin treated (right) labeled with Phospho-p38 MAP Kinase (Thr180/Tyr182) (28B10) Mouse mAb (green). Actin filaments have been labeled with Alexa Fluor Phalloidin 555 (red).


CST spho-p38 MAPK (Thr180/Tyr182) (28B10) Mouse mAb

Background

p38 MAP kinase (MAPK), also called RK (1) or CSBP (2), is the mammalian orthologue of the yeast HOG kinase that participates in a signaling cascade controlling cellular responses to cytokines and stress (1-4). Four isoforms of p38 MAPK, p38α, β, γ (also known as Erk6 or SAPK3), and δ (also known as SAPK4) have been identified. Similar to the SAPK/JNK pathway, p38 MAPK is activated by a variety of cellular stresses including osmotic shock, inflammatory cytokines, lipopolysaccharide (LPS), UV light, and growth factors (1-5). MKK3, MKK6, and SEK activate p38 MAPK by phosphorylation at Thr180 and Tyr182. Activated p38 MAPK has been shown to phosphorylate and activate MAPKAP kinase 2 (3) and to phosphorylate the transcription factors ATF-2 (5), Max (6), and MEF2 (5-8).SB203580 (4-(4-fluorophenyl)-2-(4-methylsulfinylphenyl)-5-(4-pyridyl)-imidazole) is a selective inhibitor of p38 MAPK. This compound inhibits the activation of MAPKAPK-2 by p38 MAPK and subsequent phosphorylation of HSP27 (9). SB203580 inhibits p38 MAPK catalytic activity by binding to the ATP-binding pocket, but does not inhibit phosphorylation of p38 MAPK by upstream kinases (10).

 

  1. Rouse, J. et al. (1994) Cell 78, 1027-1037.
  2. Han, J. et al. (1994) Science 265, 808-811.
  3. Lee, J.C. et al. (1994) Nature 372, 739-746.
  4. Freshney, N.W. et al. (1994) Cell 78, 1039-1049.
  5. Raingeaud, J. et al. (1995) J. Biol. Chem. 270, 7420-7426.
  6. Zervos, A.S. et al. (1995) Proc. Natl. Acad. Sci. USA 92, 10531-10534.
  7. Zhao, M. et al. (1999) Mol. Cell. Biol. 19, 21-30.
  8. Yang, S.H. et al. (1999) Mol. Cell. Biol. 19, 4028-4038.
  9. Cuenda, A. et al. (1995) FEBS Lett 364, 229-33.
  10. Kumar, S. et al. (1999) Biochem Biophys Res Commun 263, 825-31.

Application References

  • Gehani, S.S. et al. (2010) Mol Cell 39, 886-900. Applications: Western Blotting
  • Xu, P. and Derynck, R. (2010) Mol Cell 37, 551-66. Applications: Western Blotting
  • Wu, M. et al. (2009) Diabetes Metab Res Rev 25, 279-86. Applications: Western Blotting
  • Basak, C. et al. (2005) J Biol Chem 280, 4279-88. Applications: Western Blotting

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!

Companion Products

  • 9913 Phospho-p38 MAPK Pathway Sampler Kit
  • 9213 p38 MAPK Control Cell Extracts
  • 4511 Phospho-p38 MAPK (Thr180/Tyr182) (D3F9) XP® Rabbit mAb
  • 9215 Phospho-p38 MAPK (Thr180/Tyr182) (3D7) Rabbit mAb
  • 2371 p38α MAPK (7D6) Rabbit mAb
  • 9217 p38α MAPK (5F11) Mouse mAb
  • 9228 p38α MAPK (L53F8) Mouse mAb
  • 2339 p38β MAPK (C28C2) Rabbit mAb
  • 2308 p38δ MAPK (10A8) Rabbit mAb
  • 4631 Phospho-p38 MAPK (Thr180/Tyr182) (12F8) Rabbit mAb
  • 2307 p38γ MAPK Antibody
  • 9211 Phospho-p38 MAPK (Thr180/Tyr182) Antibody
  • 9212 p38 MAPK Antibody
  • 9218 p38α MAPK Antibody
  • 9214 p38δ MAPK Antibody
  • 9210 PhosphoPlus® p38 MAPK (Thr180/Tyr182) Antibody Kit
  • 9219 Phospho-p38 MAPK (Thr180/Tyr182) Mouse mAb (Sepharose Bead Conjugate)
  • 7072 Phototope®-HRP Western Blot Detection System, Anti-mouse IgG, HRP-linked Antibody
  • 7076 Anti-mouse IgG, HRP-linked Antibody
  • 7720 Prestained Protein Marker, Broad Range (Premixed Format)
  • 7727 Biotinylated Protein Ladder Detection Pack
  • 7003 20X LumiGLO® Reagent and 20X Peroxide

For Research Use Only. Not For Use In Diagnostic Procedures.

 

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