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PNGase F from Elizabethkingia miricola

  • 更新时间:  2017-12-29
  • 产品型号:  sigmaG5166
  • 简单描述
  • PNGase F from Elizabethkingia miricola
    buffered aqueous solution
详细介绍

PNGase F from Elizabethkingia miricola

Application

Used to deglycosylate protein.

Biochem/physiol Actions

Cleaves an entire glycan from a glycoprotein provided the glycosylated asparagine moiety is substituted on its amino and carboxyl terminus with a polypeptide chain.

PNGase F from Elizabethkingia miricola

Unit Definition

One unit will catalyze the release of N-linked oligosaccharides from 1 nanomole of denatured ribonuclease B in one minute at 37°C at pH 7.5 monitored by SDS-PAGE. One Sigma unit of PNGase F activity is equal to 1 IUB milliunit.

Physical form

Solution in 20 mM Tris HCl, pH 7.5, 50 mM NaCl and 1 mM EDTA

 

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