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Pharmcia原装葡聚糖凝胶G-100Sephadex G-100

  • 更新时间:  2017-12-05
  • 产品型号:  Pharmcia17-0060-01
  • 简单描述
  • Pharmcia原装葡聚糖凝胶G-100Sephadex G-100
    别名:葡聚糖凝胶G-100,
    规格:分离:肽及球形蛋白质:4000~150000;葡聚糖(线性分子):1000~100000
    包装:10克
    100克
    CAS号:9050-94-6
    保存条件:RT
详细介绍

Pharmcia原装葡聚糖凝胶G-100Sephadex G-100

MatrixCross-linked dextran
Particle Size Wet100 µm-310 µm
Partical Size Dry40 µm-120 µm
Pressure/Flow SpecificationCare must be taken to avoid compressing the gel. Do not increase the pressure beyond 0.096 bar.
Fractionation [Mr] Globular Proteins4 x 103-1.5 x 105
Fractionation [Mr] Dextrans1 x 103-1 x 105
Exclusion Limit> 1.5 x 105
AutoclavableIn wet form (pH 7.0) at 120°C for 30 min

Sephadex G-100, 100 g

Application note
Purification and renaturation of recombinant proteins produced in Escherichia coli as inclusion bodies Summary

Expression of eukaryotic genes in the cytoplasm of Escherichia coli often leads to the production and accumulation of the recombinant protein (r-protein) to levels of total cell protein that are higher than when secreted, in some instances by as much as 25%. The r-proteins so produced are frequently in the form of insoluble aggregates, so-called inclusion bodies (IBs). The IBs contain reduced and denatured proteins lacking biological activity. Their sizes and densities are also distinct from most of the bacterial proteins, which facilitates their separation from intracellular E. coli proteins and cell debris by slow speed centrifugation. A general protocol for the extraction and purification of IBs from E. coli cells is shown. The purified IBs are usually dissolved in 6 M guanidine hydrochloride or 8 M urea at near neutral pH prior to re-naturation and purification of the r-protein. The most commonly used procedure for refolding of such de-natured r-proteins is slow dialysis, or dilution into a buffer of near neutral pH. This result in a significant dilution of the r-protein, typically to a few mg of r-protein per mL of solution, with the formation of precipitated proteins being often unavoidable. Alternative purification and/or refolding procedures using gel filtration, ion exchange or hydrophobic interaction chromatography (HIC) have been developed to circumvent such problems. These are outlined briefly in this Application Note. Close

Handbook
Size Exclusion Chromatography: Principles and Methods Summary

This handbook describes the use of SEC for the purification and separation of biomolecules, with a focus on practical information for obtaining the best results. The media available, selection criteria and examples with detailed instructions for the most common applications are included, as well as the theoretical principles behind the technique. The first step towards a successful separation is to select the correct medium and this handbook focuses on the most up-to-date SEC media and prepacked columns. Close

Magazine
Upstream Downstream issue 2

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Material Safety Data Sheet
Sephadex™ G-100, 100 g

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Sephadex™ G-100, 100 g

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Pharmcia原装葡聚糖凝胶G-100Sephadex G-100

17-0060-01葡聚糖凝胶G-100Sephadex G-100
17-0050-01葡聚糖凝胶G-75Sephadex G-75
17-0043-01葡聚糖凝胶G-50Sephadex G-50
17-0033-01葡聚糖凝胶G-25[中]Sephadex G-25
17-0020-01葡聚糖凝胶G-15Sephadex G-15
17-0010-01葡聚糖凝胶G-10Sephadex G-10
B-2261荧光染糖[NO.33342]bisBenzimide H 33342 trihydrochloride
17-0170-01DEAE葡聚糖凝胶A-25DEAE Sephadex A-25
17-0170-02DEAE葡聚糖凝胶A-25DEAE Sephadex A-25
17-0180-01DEAE葡聚糖凝胶A-50DEAE Sephadex A-50
17-0180-02DEAE葡聚糖凝胶A-50DEAE Sephadex A-50
G-9382明胶Gelatin
L-5125N-十二烷基肌氨酸钠N-Lauroylsarcosine sodium salt
L-5125N-十二烷基肌氨酸钠N-Lauroylsarcosine sodium salt
0719N-十二烷基肌氨酸钠N-Lauroylsarcosine sodium salt
0719N-十二烷基肌氨酸钠N-Lauroylsarcosine sodium salt
T-87683,3'5,5'-四甲基联苯胺二盐酸TMB 2Hcl
T-87683,3'5,5'-四甲基联苯胺二盐酸TMB 2Hcl
07593,3'5,5'-四甲基联苯胺二盐酸TMB 2Hcl
07593,3'5,5'-四甲基联苯胺二盐酸TMB 2Hcl
E-2129埃文斯蓝[伊文斯蓝]Evans Blue
E-2129埃文斯蓝[伊文斯蓝]Evans Blue
19555埃文斯蓝[伊文斯蓝]Evans Blue
19555埃文斯蓝[伊文斯蓝]Evans Blue
0335蔗糖Sucrose
21938蔗糖Sucrose
F-0127D-果糖D-(-)Fructose
0226D-果糖D-(-)Fructose
0528结晶紫crystal violet
0671D-核糖D-Ribose
J392棉子糖D-Raffinose
P3504丽春红SPonceau S
0860丽春红SPonceau S
C-2037细胞色素C[牛心]Cytochromes C(Bovine Heart)
C-7752细胞色素C[马心]Cytochromes C(Horse Heart)
0322 EDTA
0322 EDTA
D-4551N,N-二甲基甲酰胺N,N-Dimethylformamide
0464N,N-二甲基甲酰胺N,N-Dimethylformamide
T-9250胸腺嘧啶核苷Thymidine
T-9250胸腺嘧啶核苷Thymidine
22305胸腺嘧啶核苷Thymidine
22305胸腺嘧啶核苷Thymidine
P-3250木瓜蛋白酶Papain from papaya latex
P-3250木瓜蛋白酶Papain from papaya latex
G-3632硫酸庆大霉素Gentamicin sulfate salt
G-3632硫酸庆大霉素Gentamicin sulfate salt
0304硫酸庆大霉素Gentamicin sulfate salt
0304硫酸庆大霉素Gentamicin sulfate salt
C-7698[环已亚胺]Cycloheximide
C-7698[环已亚胺]Cycloheximide
D-1756地塞米松(氢化可的松)Dexamethasone
D-1756地塞米松(氢化可的松)Dexamethasone
A-7506维生素C[抗球血酸钠]L-Ascorbic acid[Vitamin C]
A-7506维生素C[抗球血酸钠]L-Ascorbic acid[Vitamin C]
0764维生素C[抗球血酸钠]L-Ascorbic acid[Vitamin C]
0764维生素C[抗球血酸钠]L-Ascorbic acid[Vitamin C]
 *******维生素C[抗球血酸钠]L-Ascorbic acid[Vitamin C]
 *******氯化锂Lithium chloride
0416氯化锂Lithium chloride
22178纤维素酶Cellulase from Aspergillus niger
****纤维素酶Cellulase from Aspergillus niger
M-8410矿物油[石腊油]Mineral oil
J217矿物油[石腊油]Mineral oil
C-7880L-半胱氨酸盐酸盐L-Cysteine hydrochloride monohydrate
C-7880L-半胱氨酸盐酸盐L-Cysteine hydrochloride monohydrate
0206L-半胱氨酸盐酸盐L-Cysteine hydrochloride monohydrate
0206L-半胱氨酸盐酸盐L-Cysteine hydrochloride monohydrate
E-4378乙二醇-双-(2-氨基乙基)四乙酸Ethylene glycol-bis(2-aminoethylether)-N,N,N′,N′-tetraacetic acid[EGTA]
0732乙二醇-双-(2-氨基乙基)四乙酸Ethylene glycol-bis(2-aminoethylether)-N,N,N′,N′-tetraacetic acid[EGTA]
T-88772,3,5-氯化三苯基四氮唑2,3,5-Triphenyltetrazolium chloride[TTC]
07652,3,5-氯化三苯基四氮唑2,3,5-Triphenyltetrazolium chloride[TTC]
B-0252硼酸Boric acid
0588硼酸Boric acid
P-3640聚乙二醇 3350PEG 3350
P-3640聚乙二醇 3350PEG 3350
817006聚乙二醇 4000PEG 4000
817006聚乙二醇 4000PEG 4000
807491聚乙二醇 6000PEG 6000
807491聚乙二醇 6000PEG 6000
0159聚乙二醇 8000PEG 8000
0159聚乙二醇 8000PEG 8000
81285聚乙二醇 12000PEG 12000
81285聚乙二醇 12000PEG 12000
818897聚乙二醇 20000PEG 20000
818897聚乙二醇 20000PEG 20000
K-3378激动素[6-呋喃氨基嘌呤]6-Furfuryl Aminopurine(Kinetin)[KT]
K-3378激动素[6-呋喃氨基嘌呤]6-Furfuryl Aminopurine(Kinetin)[KT]
17980激动素[6-呋喃氨基嘌呤]6-Furfuryl Aminopurine(Kinetin)[KT]
17980激动素[6-呋喃氨基嘌呤]6-Furfuryl Aminopurine(Kinetin)[KT]
N-3503制霉菌素Nystatin
N-3503制霉菌素Nystatin
19770制霉菌素Nystatin
19770制霉菌素Nystatin
D-56373,3'-二氨基联苯胺3,3′-Diaminobenzidine tetrahydrochloride hydrate[DAB]
D-56373,3'-二氨基联苯胺3,3′-Diaminobenzidine tetrahydrochloride hydrate[DAB]
04303,3'-二氨基联苯胺3,3′-Diaminobenzidine tetrahydrochloride hydrate[DAB]
04303,3'-二氨基联苯胺3,3′-Diaminobenzidine tetrahydrochloride hydrate[DAB]
I-2886吲哚-3-乙酸3-Indoleacetic acid[IAA]
I-2886吲哚-3-乙酸3-Indoleacetic acid[IAA]
17550吲哚-3-乙酸3-Indoleacetic acid[IAA]
17550吲哚-3-乙酸3-Indoleacetic acid[IAA]
A-5006L-精氨酸L-Arginine
A-5006L-精氨酸L-Arginine
11490L-精氨酸L-Arginine
11490L-精氨酸L-Arginine
M-9625L-甲硫氨酸[L-蛋氨酸]L-Methionine
M-9625L-甲硫氨酸[L-蛋氨酸]L-Methionine
18915L-甲硫氨酸[L-蛋氨酸]L-Methionine
18915L-甲硫氨酸[L-蛋氨酸]L-Methionine
G-8270D-葡萄糖[无水]D-(+)-Glucose
0188D-葡萄糖[无水]D-(+)-Glucose
F-9037去离子甲酰胺Formamide,Deionized
0606去离子甲酰胺Formamide,Deionized

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